Generating Genetically Modified Mice: A Decision Guide

  • Ivo J. HuijbersEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1642)


The generation of a new genetically modified mouse strain is a big hurdle to take for many researchers. It is often unclear which steps and decisions have to be made prior to obtaining the desired mouse model. This review aims to help researchers by providing a decision guide that answers the essential questions that need to be asked before generating the most suitable genetically modified mouse line in the most optimal timeframe. The review includes the latest technologies in both the stem cell culture and gene editing tools, particularly CRISPR/Cas9, and provides compatibility guidelines for selecting among the different types of genetic modifications that can be introduced in the mouse genome and the various routes for introducing these modifications into the mouse germline.

Key words

Transgenesis Genetic engineering Mouse models CRISPR/Cas9 Embryonic stem cell Zygote Gene targeting Recombinase Transposon GEMM-ESC 


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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Mouse Clinic for Cancer and Aging, The Netherlands Cancer InstituteAmsterdamThe Netherlands

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