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Deconstructing the Metabolic Networks of Oncogenic Signaling Using Targeted Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

  • George PoulogiannisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1636)

Abstract

Metabolic reprogramming is recognized as an emerging hallmark of oncogenic signaling and cancer development. Hence the need to identify novel quantitative analytical platforms for studying metabolism in vitro and in vivo has dramatically increased. Here, we describe the experimental workflow for a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach involving positive/negative ion switching to analyze >250 metabolites of central carbon metabolism, nucleotides, and amino acids.

Key words

Liquid chromatography-tandem mass spectrometry Targeted metabolomics Cancer metabolism 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Division of Cancer BiologyThe Institute of Cancer ResearchLondonUK

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