Capture and Genetic Analysis of Circulating Tumor Cells Using a Magnetic Separation Device (Magnetic Sifter)
- 1.6k Downloads
Circulating tumor cells (CTCs) are currently widely studied for their potential application as part of a liquid biopsy. These cells are shed from the primary tumor into the circulation, and are postulated to provide insight into the molecular makeup of the actual tumor in a minimally invasive manner. However, they are extremely rare in blood, with typical concentrations of 1–100 in a milliliter of blood; hence, a need exists for a rapid and high-purity method for isolating CTCs from whole blood. Here, we describe the application of a microfabricated magnetic sifter toward isolation of CTCs from whole blood at volumetric flow rates of 10 mL/h, along with the use of a PDMS-based nanowell system for single-cell gene expression profiling. This method allows rapid isolation of CTCs and subsequent integration with downstream genetic profiling methods for clinical applications such as targeted therapy, therapy monitoring, or further biological studies.
Key wordsMagnetic cell separation Circulating tumor cell Liquid biopsy Genetic analysis Magnetic sifter Nanowell array
This work was supported by the U.S. National Institutes of Health (NIH) Awards U54CA151459 (Center for Cancer Nanotechnology Excellence and Translation) and R21CA185804.
- 5.Park S-m, Wong DJ, Ooi CC, Kurtz DM, Vermesh O, Aalipour A et al (2016) Molecular profiling of single circulating tumor cells from lung cancer patients. Proc Natl Acad Sci U S A 113(52):E8379–E8386Google Scholar
- 6.Dimov IK, Lu R, Lee EP, Seita J, Sahoo D, Park S-m et al (2014) Discriminating cellular heterogeneity using microwell-based RNA cytometry. Nat Commun 5:Article ID 3451Google Scholar
- 7.Park S-m, Lee JY, Hong S, Lee SH, Dimov IK, Lee H et al (2016) Dual transcript and protein quantification in a massive single cell array. Lab Chip 16(19):3682–3688Google Scholar
- 8.Park S-m, Wong DJ, Ooi CC, Nesvet JC, Nair VS, Wang SX et al (2016) Multigene profiling of single circulating tumor cells. Mol Cell Oncol 4 (2):E1289295Google Scholar