Preparation of a Conditional RNA Switch
RNA has gained great interest for use in biomedical and therapeutic applications. This is due in part to RNA’s ability to perform multiple functions, including the regulation of endogenously expressed genes. However, the ability of RNA based drugs to distinguish target diseased cells from healthy tissue remains challenging. Here we present methods for the production of a recently developed conditional RNA switch that releases a Dicer substrate RNA in response to interaction with a specific RNA biomarker.
Key wordsRNA Toehold Nanotechnology Nanomedicine RNase H RNA switch Conditional RNA
This work has been funded in whole or in part with Federal funds from the Frederick National Laboratory for Cancer Research, National Institutes of Health, under Contract No. HHSN261200800001E. This research was supported [in part] by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government.
- 17.Hochrein LM, Schwarzkopf M, Shahgholi M, Yin P, Pierce NA (2013) Conditional Dicer substrate formation via shape and sequence transduction with small conditional RNAs. J Am Chem Soc 135(46):17322–17330Google Scholar