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Preparation of a Conditional RNA Switch

  • Paul Zakrevsky
  • Lorena Parlea
  • Mathias Viard
  • Eckart Bindewald
  • Kirill A. Afonin
  • Bruce A. ShapiroEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1632)

Abstract

RNA has gained great interest for use in biomedical and therapeutic applications. This is due in part to RNA’s ability to perform multiple functions, including the regulation of endogenously expressed genes. However, the ability of RNA based drugs to distinguish target diseased cells from healthy tissue remains challenging. Here we present methods for the production of a recently developed conditional RNA switch that releases a Dicer substrate RNA in response to interaction with a specific RNA biomarker.

Key words

RNA Toehold Nanotechnology Nanomedicine RNase H RNA switch Conditional RNA 

Notes

Acknowledgments

This work has been funded in whole or in part with Federal funds from the Frederick National Laboratory for Cancer Research, National Institutes of Health, under Contract No. HHSN261200800001E. This research was supported [in part] by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Paul Zakrevsky
    • 1
  • Lorena Parlea
    • 1
  • Mathias Viard
    • 5
    • 6
  • Eckart Bindewald
    • 2
  • Kirill A. Afonin
    • 3
    • 4
  • Bruce A. Shapiro
    • 1
    Email author
  1. 1.RNA Structure and Design Section, RNA Biology LaboratoryNational Cancer Institute, National Institutes of HealthFrederickUSA
  2. 2.Frederick National Laboratory for Cancer ResearchLeidos Biomedical ResearchFrederickUSA
  3. 3.Nanoscale Science Program, Department of ChemistryUniversity of North Carolina at CharlotteCharlotteUSA
  4. 4.The Center for Biomedical Engineering and ScienceUniversity of North Carolina at CharlotteCharlotteUSA
  5. 5.RNA Structure and Design Section, RNA Biology LaboratoryCenter for Cancer Research, National Cancer Institute, National Institutes of HealthFrederickUSA
  6. 6.Leidos Biomedical Research Inc.Center for Cancer Research, National Cancer Institute, National Institutes of HealthFrederickUSA

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