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Chitosan Nanoparticles for miRNA Delivery

  • Merve Denizli
  • Burcu Aslan
  • Lingegowda S. Mangala
  • Dahai Jiang
  • Cristian Rodriguez-Aguayo
  • Gabriel Lopez-Berestein
  • Anil K. Sood
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1632)

Abstract

RNA interference techniques represent a promising strategy for therapeutic applications. In addition to small interfering RNA-based approaches, which have been widely studied and translated into clinical investigations, microRNA-based approaches are attractive owing to their “one hit, multiple targets” concept. To overcome challenges with in vivo delivery of microRNAs related to stability, cellular uptake, and specific delivery, our group has developed and characterized chitosan nanoparticles for nucleotide delivery. This platform allows for robust target modulation and antitumor activity following intravenous administration.

Key words

RNA interference miR-34a microRNA delivery Chitosan nanoparticles Prostate cancer 

Notes

Acknowledgments

Portions of this work were supported by the NIH (CA016672, CA109298, P50 CA083639, P50 CA098258, UH3 TR000943), the Ovarian Cancer Research Fund, Inc. (Program Project Development Grant), the Blanton-Davis Ovarian Cancer Research Program, the RGK Foundation, and the Gilder Foundation.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Merve Denizli
    • 1
  • Burcu Aslan
    • 1
    • 2
  • Lingegowda S. Mangala
    • 2
    • 3
  • Dahai Jiang
    • 2
    • 3
  • Cristian Rodriguez-Aguayo
    • 1
    • 2
  • Gabriel Lopez-Berestein
    • 1
    • 2
    • 5
  • Anil K. Sood
    • 2
    • 3
    • 4
    • 5
  1. 1.Department of Experimental TherapeuticsThe University of Texas MD Anderson Cancer CenterHoustonUSA
  2. 2.Center for RNA Interference and Non-coding RNAThe University of Texas MD Anderson Cancer CenterHoustonUSA
  3. 3.Department of Gynecologic Oncology and Reproductive MedicineThe University of Texas MD Anderson Cancer CenterHoustonUSA
  4. 4.Program in Cancer Biology and Cancer MetastasisThe University of Texas Graduate School of Biomedical Sciences at HoustonHoustonUSA
  5. 5.Department of Cancer BiologyThe University of Texas MD Anderson Cancer CenterHoustonUSA

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