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ChIP-Seq Analysis for Identifying Genome-Wide Histone Modifications Associated with Stress-Responsive Genes in Plants

Part of the Methods in Molecular Biology book series (MIMB,volume 1631)

Abstract

Histone modifications represent the crux of epigenetic gene regulation essential for most biological processes including abiotic stress responses in plants. Thus, identification of histone modifications at the genome-scale can provide clues for how some genes are ‘turned-on’ while some others are “turned-off” in response to stress. This chapter details a step-by-step protocol for identifying genome-wide histone modifications associated with stress-responsive gene regulation using chromatin immunoprecipitation (ChIP) followed by sequencing of the DNA (ChIP-seq).

Key words

  • Abiotic stresses
  • Chromatin immunoprecipitation (ChIP)
  • Epigenome
  • Gene regulation
  • Histone modifications

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Acknowledgments

The work in RS laboratory was supported by the Oklahoma Center for Advancement of Science and Technology and Oklahoma Agricultural Experiment Station. AM acknowledges the Stevens endowed Chair professorship in Agricultural Biotechnology of DASNR, OSU.

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Correspondence to Ramanjulu Sunkar .

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Li, G., Jagadeeswaran, G., Mort, A., Sunkar, R. (2017). ChIP-Seq Analysis for Identifying Genome-Wide Histone Modifications Associated with Stress-Responsive Genes in Plants. In: Sunkar, R. (eds) Plant Stress Tolerance. Methods in Molecular Biology, vol 1631. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7136-7_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7136-7_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7134-3

  • Online ISBN: 978-1-4939-7136-7

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