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Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease

  • Yasunori Sasakura
  • Keita Yoshida
  • Nicholas Treen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1630)

Abstract

The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods.

Key words

Ascidian Ciona intestinalis Genome editing TALEN Knockout Electroporation 

Notes

Acknowledgments

We would like to thank Drs. Hiroshi Ochiai, Tetsushi Sakuma, and Takashi Yamamoto for their help to support our researches. This study was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) and Ministry of Education, Culture, Sports, Science and Technology (MEXT) to Y.S. Y.S. was supported by the Toray Science and Technology Grant. Further support was provided by grants from the National Bioresource Project.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Yasunori Sasakura
    • 1
  • Keita Yoshida
    • 1
  • Nicholas Treen
    • 1
  1. 1.Shimoda Marine Research CenterUniversity of TsukubaShimodaJapan

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