Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods.
Key wordsAscidian Ciona intestinalis Genome editing TALEN Knockout Electroporation
We would like to thank Drs. Hiroshi Ochiai, Tetsushi Sakuma, and Takashi Yamamoto for their help to support our researches. This study was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) and Ministry of Education, Culture, Sports, Science and Technology (MEXT) to Y.S. Y.S. was supported by the Toray Science and Technology Grant. Further support was provided by grants from the National Bioresource Project.
- 6.Satou Y, Mineta K, Ogasawara M et al (2008) Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and populations. Genome Res 9:R152Google Scholar