Genome Editing Mediated by Primordial Germ Cell in Chicken

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1630)

Abstract

Rapid development of genome editing technology has facilitated the studies on exploring specific gene functions and establishment of model animals. In livestock, the technology has contributed to create high value in industry fields, e.g., enhancing productivity or acquiring the resistance against disease. Meanwhile, genome editing in avian species has been emphasized because of their applicable possibilities in terms of highly productive chickens, disease-controlled avian lines, and development of novel biological models. Induction of exogenous gene using virus system or transposition in chicken primordial germ cells (PGCs) has been widely used for producing transgenic chicken, and recently developed programmable genome editing (PGE) technologies such as tale transcription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated (Cas9) are expected to maximize the applicable potentials of avian species. In this regard, this chapter will cover the methods for producing genome-edited chicken by piggyBac transposition and gene targeting technology, TALEN, and CRISPR/Cas9.

Key words

Genome editing Chicken Primordial germ cell piggyBac transposition TALEN CRISPR/Cas9 
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Notes

Acknowledgment

This work was supported by a National Research Foundation of Korea (NRF) grant, funded by the Korean government (MSIP; No. 2015R1A3A2033826), and the Next-Generation BioGreen 21 Program Grant PJ008142, Rural Development Administration, Republic of Korea.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Agricultural Biotechnology and Research Institute of Agriculture and Life SciencesCollege of Agriculture and Life Sciences, Seoul National UniversitySeoulKorea

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