Ebolaviruses pp 109-117 | Cite as

Modeling Ebolavirus Budding with Virus Like Particles

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1628)

Abstract

About 15 years ago, several groups initially described the release of virus like particles (VLPs) upon expression of Ebola virus VP40 in mammalian cells. Further development of the protocol later allowed for the dissection of the Ebola virus budding mechanism and for the identification of critical VP40 residues involved in this process. VLPs are now produced routinely in several laboratories as a tool to study virus entry or egress and have even been proposed as vaccine candidates against Ebola virus disease. Here we described protocols for the production and the analysis of Ebola virus VLP release.

Key words

Ebola Ebolavirus VP40 Viruslike particles 
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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.INSERM U1111—CNRS UMR5308, Centre International de Recherche en Infectiologie (CIRI)Université Claude Bernard Lyon 1, Ecole Normale Supérieure de LyonLyonFrance
  2. 2.UBIVE, Institut Pasteur, CIRILyonFrance

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