A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography
In-gel zymography is a commonly employed tool to identify active enzymes in a quantitative and qualitative manner. In this work, apart from the incorporation of substrate which is traditionally employed in zymography, the identification of collagenase by incubation of the enzyme resolved on a polyacrylamide gel with substrate solution is described. The two methods are quite fast and result in specific detection of bacterial collagenase.
Key wordsCollagen Collagenase Matrix metalloproteinase Zymography
Partial financial support from Department of Science and Technology is acknowledged. The authors thank K. Thanzami for her contribution to the work described here.