Generation and Analysis of Chromosomal Contact Maps of Bacteria
This methods article described a protocol aiming at generating chromosome contact maps of bacterial species using a genome-wide derivative of the chromosome conformation capture (3C) technique. The approach is readily applicable on a broad variety of gram + and gram-bacterial species. It describes and addresses known caveats and technicalities associated with the technique, and should be of interest to any laboratory interested to perform a multiscale analysis of the genome structure of its species of interest.
Key wordsChromosome conformation capture, Hi-C 3C Genome organization Nucleoid
This research was supported by funding to R.K. from the European Research Council under the seventh Framework Program (FP7/2007-2013, ERC grant agreement 260822) and from Agence Nationale pour la Recherche (HiResBaCS ANR-15-CE11-0023).
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