Mass Spectrometry-Based Serum Proteomics for Biomarker Discovery and Validation
Blood protein measurements are used frequently in the clinic in the assessment of patient health. Nevertheless, there remains the need for new biomarkers with better diagnostic specificities. With the advent of improved technology for bioanalysis and the growth of biobanks including collections from specific disease risk cohorts, the plasma proteome has remained a target of proteomics research toward the characterization of disease-related biomarkers. The following protocol presents a workflow for serum/plasma proteomics including details of sample preparation both with and without immunoaffinity depletion of the most abundant plasma proteins and methodology for selected reaction monitoring mass spectrometry validation.
Key wordsSerum/plasma Label-free quantification Selected reaction monitoring Proteomics Mass spectrometry
The National Technology Agency of Finland (Finland Distinguished Professor Programme, grant 40398/11), the Academy of Finland (Centre of Excellence in Molecular Systems Immunology and Physiology Research, grant 250114), JDRF, the Sigrid Jusélius Foundation, and Biocentre Finland (Turku Proteomics Facility) are thanked for their financial support.
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