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Serum/Plasma Proteomics pp 451–466Cite as

Mass Spectrometry-Based Serum Proteomics for Biomarker Discovery and Validation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1619))

Abstract

Blood protein measurements are used frequently in the clinic in the assessment of patient health. Nevertheless, there remains the need for new biomarkers with better diagnostic specificities. With the advent of improved technology for bioanalysis and the growth of biobanks including collections from specific disease risk cohorts, the plasma proteome has remained a target of proteomics research toward the characterization of disease-related biomarkers. The following protocol presents a workflow for serum/plasma proteomics including details of sample preparation both with and without immunoaffinity depletion of the most abundant plasma proteins and methodology for selected reaction monitoring mass spectrometry validation.

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Acknowledgment

The National Technology Agency of Finland (Finland Distinguished Professor Programme, grant 40398/11), the Academy of Finland (Centre of Excellence in Molecular Systems Immunology and Physiology Research, grant 250114), JDRF, the Sigrid Jusélius Foundation, and Biocentre Finland (Turku Proteomics Facility) are thanked for their financial support.

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Authors and Affiliations

  1. Turku Centre for Biotechnology, University of Turku, Turku, Finland

    Santosh D. Bhosale, Robert Moulder, Petri Kouvonen, Riitta Lahesmaa & David R. Goodlett

  2. Department of Pharmaceutical Science, University of Maryland, 20 North Pine Street, Baltimore, MD, 21201, USA

    David R. Goodlett

Authors
  1. Santosh D. Bhosale
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  2. Robert Moulder
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  3. Petri Kouvonen
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  4. Riitta Lahesmaa
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  5. David R. Goodlett
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Corresponding author

Correspondence to David R. Goodlett .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia

    David W. Greening

  2. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia

    Richard J. Simpson

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Bhosale, S.D., Moulder, R., Kouvonen, P., Lahesmaa, R., Goodlett, D.R. (2017). Mass Spectrometry-Based Serum Proteomics for Biomarker Discovery and Validation. In: Greening, D., Simpson, R. (eds) Serum/Plasma Proteomics. Methods in Molecular Biology, vol 1619. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7057-5_31

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  • DOI: https://doi.org/10.1007/978-1-4939-7057-5_31

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7056-8

  • Online ISBN: 978-1-4939-7057-5

  • eBook Packages: Springer Protocols

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