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MALDI-MSI of Lipids in Human Skin

  • Philippa J. HartEmail author
  • Malcolm R. Clench
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1618)

Abstract

Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is now a well-established technique for imaging analysis of sectioned biological tissues. One of the growing areas of interest is in the analysis of skin. MALDI-MSI can provide a wealth of information from within sections of skin. This includes information on the distribution of pharmaceuticals following topical treatments, through to the examination of the composition of different skin layers and studies of proteomic, lipidomic, and metabolomic responses to disease, wounds, and external stimuli. Here, we describe the handling procedures, preparatory treatment, and mass spectrometry setup required for the MALDI MSI analysis of lipids within human skin samples.

Key words

MALDI MS MSI Imaging Skin Lipids 

Notes

Acknowledgments

This work was supported by the Biomolecular Sciences Research Centre of Sheffield Hallam University.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Waters CorporationWilmslowUK
  2. 2.Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research CentreSheffield Hallam UniversitySheffieldUK

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