Protocol

Diagnostic Bacteriology

Volume 1616 of the series Methods in Molecular Biology pp 137-146

Date:

Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization

  • Silvia FonteneteAffiliated withLEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Portoi3s Instituto de Investigação e Inovação em Saúde, Universidade do PortoIPATIMUP, Institute of Molecular Pathology and Immunology of the University of PortoNucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern DenmarkICBAS, Institute of Biomedical Sciences Abel Salazar, University of PortoEpithelial Cell Biology Group, Cancer Cell Biology Program, Spanish National Cancer Research Centre
  • , Marina LeiteAffiliated withi3s Instituto de Investigação e Inovação em Saúde, Universidade do PortoIPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto
  • , Ceu FigueiredoAffiliated withi3s Instituto de Investigação e Inovação em Saúde, Universidade do PortoIPATIMUP, Institute of Molecular Pathology and Immunology of the University of PortoFMUP, Faculty of Medicine of the University of Porto
  • , Paul CosAffiliated withLaboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp
  • , Nuno F. AzevedoAffiliated withLEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto Email author 

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Abstract

In this chapter, we describe a fluorescence in vivo hybridization (FIVH) protocol, using nucleic acid probes, for the detection of the bacterium Helicobacter pylori in the gastric mucosa of an infected C57BL/6 mouse model. This protocol should be easily extended to other microorganisms not only as a way to identify in vivo important microorganisms and their patterns of distribution within specific or at different anatomic sites, but also to better understand interaction mechanisms involving the microbiome and the human body.

Key words

Diagnostics Microbiology Nucleic acids In vivo Helicobacter pylori Fish FIVH