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Protein–Protein Interactions: Pull-Down Assays

  • Arthur Louche
  • Suzana P. Salcedo
  • Sarah Bigot
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1615)

Abstract

Determining protein partners is an essential step toward understanding protein function and identifying relevant biological pathways. Many methods exist for investigating protein–protein interactions. The pull-down assay is an in vitro technique used to detect physical interactions between two or more proteins and an invaluable tool for confirming a predicted protein–protein interaction or identifying novel interacting partners. This method typically involves the use of affinity purification with various wash and elution steps. In this chapter, we describe how an interaction between two purified bacterial proteins or between bacterial and eukaryotic proteins can be detected by pull-down experiments.

Key words

Pull-down Protein–protein interactions Tagged protein Affinity purification 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Arthur Louche
    • 1
  • Suzana P. Salcedo
    • 1
  • Sarah Bigot
    • 1
  1. 1.Molecular Microbiology and Structural Biochemistry, CNRS UMR 5086Université Lyon 1, Institut de Biologie et Chimie des ProtéinesLyon Cedex 07France

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