3D Anastomosed Microvascular Network Model with Living Capillary Networks and Endothelial Cell-Lined Microfluidic Channels

Part of the Methods in Molecular Biology book series (MIMB, volume 1612)


This protocol describes detailed practical procedures for generating 3D intact and perfusable microvascular network that connects to microfluidic channels without appreciable leakage. This advanced 3D microvascular network model incorporates different stages of vascular development including vasculogenesis, endothelial cell (EC) lining, sprouting angiogenesis, and anastomosis in sequential order. The capillary network is first induced via vasculogenesis in a middle tissue chamber and then EC linings along the microfluidic channel on either side serve as artery and vein. The anastomosis is then induced by sprouting angiogenesis to facilitate tight interconnection between the artery/vein and the capillary network. This versatile device design and its robust construction methodology establish a physiological microcirculation transport model of interconnected perfused vessels from artery to vascularized tissue to vein.

Key words

3D microvascular network Microfluidic chip Vasculogenesis EC lining Sprouting angiogenesis Anastomosis Non-physiological leakage Organ-on-a-chip 



This work was supported by grants from the National Institutes of Health: UH3 TR00048 and PQD5 CA180122. C.C.W.H. receives support from the Chao Family Comprehensive Cancer Center (CFCCC) through an NCI Center Grant award P30A062203. X.W. receives support from National Natural Science Foundation of China (No. 31600781). We would also like to thank the permission of The Royal Society of Chemistry (RSC) for reproduction of materials from Lab on a Chip journal.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Micro/Nano ElectronicsShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Department of Molecular Biology and BiochemistryUniversity of CaliforniaIrvineUSA
  3. 3.Department of Biomedical EngineeringWashington University in St. LouisSaint LouisUSA
  4. 4.Department of Biomedical EngineeringUniversity of CaliforniaIrvineUSA
  5. 5.Edwards Lifesciences Center for Advanced Cardiovascular TechnologyUniversity of CaliforniaIrvineUSA
  6. 6.Department of Mechanical and Aerospace EngineeringUniversity of CaliforniaIrvineUSA

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