Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources

  • Marius Schmidt
Part of the Methods in Molecular Biology book series (MIMB, volume 1607)


Time-resolved macromolecular crystallography unifies protein structure determination with chemical kinetics. With the advent of fourth generation X-ray sources the time-resolution can be on the order of 10–40 fs, which opens the ultrafast time scale to structure determination. Fundamental motions and transitions associated with chemical reactions in proteins can now be observed. Moreover, new experimental approaches at synchrotrons allow for the straightforward investigation of all kind of reactions in biological macromolecules. Here, recent developments in the field are reviewed.

Key words

Time-resolved macromolecular crystallography Time-resolved serial femtosecond crystallography Structure based enzymology Chemical kinetics 



M.S. thanks Vukica Šrajer for reading, and commenting on, an earlier version of the manuscript. This work is supported by the BioXFEL Science and Technology Center (NSF grant 1231306).


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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Kenwood Interdisciplinary Research Complex, Physics DepartmentUniversity of Wisconsin-MilwaukeeMilwaukeeUSA

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