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Serial Synchrotron X-Ray Crystallography (SSX)

  • Kay DiederichsEmail author
  • Meitian Wang
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1607)

Abstract

Prompted by methodological advances in measurements with X-ray free electron lasers, it was realized in the last two years that traditional (or conventional) methods for data collection from crystals of macromolecular specimens can be complemented by synchrotron measurements on microcrystals that would individually not suffice for a complete data set. Measuring, processing, and merging many partial data sets of this kind requires new techniques which have since been implemented at several third-generation synchrotron facilities, and are described here. Among these, we particularly focus on the possibility of in situ measurements combined with in meso crystal preparations and data analysis with the XDS package and auxiliary programs.

Key words

Serial synchrotron crystallography (SSX) Microcrystal Lipidic cubic phase (LCP) In meso in situ Room temperature (RT) Cryogenic temperature Data collection Data quality Merging XDS XSCALE 
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Notes

Acknowledgments

We thank Greta Assmann, Wolfgang Brehm, Martin Caffrey, Chia-Ying Huang, Vincent Olieric, Ezequiel Panepucci, Rangana Warshamanage, and all other members of the groups at the Swiss Light Source (Paul-Scherrer-Institute, Villigen, Switzerland), Trinity College (Dublin, Ireland) and University of Konstanz (Konstanz, Germany) for discussions and their contributions toward developing the methodology. We also thank Aaron Finke and Martin Caffrey for proofreading the manuscript and Rangana Warshamanage and Chia-Ying Huang for preparing the figures.

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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of BiologyUniversität KonstanzKonstanzGermany
  2. 2.Swiss Light SourcePaul Scherrer InstituteVilligenSwitzerland

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