Abstract
Identification of cell moieties involved in viral binding and internalization is essential since their expression would render a cell susceptible. Further steps that allow the uncoating of the viral particle at the right subcellular localization have been intensively studied. These “entry” steps could determine cell permissiveness and often define tissue and host tropism. Therefore applying the right and, when possible, straightforward experimental approaches would shorten avenues to the complete knowledge of this first and key step of any viral life cycle. Mammarenaviruses are enveloped viruses that enter the host cell via receptor-mediated endocytosis. In this chapter we present a set of customized experimental approaches and tools that were used to describe the entry of Junín virus (JUNV), and other New World mammarenavirus members, into mammalian cells.
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References
Cordo SM, Cesio y AM, Candurra NA (2005) Polarized entry and release of Junín virus, a new world arenavirus. J Gen Virol 86:1475–1479
Martinez MG, Cordo SM, Candurra NA (2008) Involvement of cytoskeleton in Junín virus entry. Virus Res 138:17–25
Martinez MG, Cordo SM, Candurra NA (2007) Characterization of Junín arenavirus cell entry. J Gen Virol 88:1776–1784
Martinez MG, Forlenza MB, Candurra NA (2009) Involvement of cellular proteins in Junín arenavirus entry. Biotechnol J 4:866–870
Contigiani MS, Sabattini MS (1977) Virulencia diferencial de cepas de virus Junín por marcadores biológicos en ratones y cobayos. Medicina (B Aires) 37:244–251
Martinez MG, Bialecki MA, Belouzard S, Cordo SM, Candurra NA, Whittaker GR (2013) Utilization of human DC-SIGN and L-SIGN for entry and infection of host cells by the new world arenavirus, Junín virus. Biochem Biophys Res Commun 441:612–617
Damonte EB, Mersich SE, Candurra NA (1994) Intracellular processing and transport of Junín virus glycoproteins influences virion infectivity. Virus Res 34:317–326
Sanchez A, Pifat D, Kenyon RH, Peters CJ, McCormick JB, Kiley MP (1989) Junín virus monoclonal antibodies: characterization and cross-reactivity with other arenaviruses. J Gen Virol 70:1125–1132
Damonte EB, Mersich SE, Candurra NA, Coto CE (1986) Cross reactivity between Junín and Tacaribe viruses as determined by neutralization test and immunoprecipitation. Med Microbiol Immunol 175:85–88
Castilla V, Mersich SE, Damonte EB (1991) Lysosomotropic compounds inhibiting the multiplication of Junín virus. Rev Argent Microbiol 23:86–89
Benmerah A, Bayrou M, Cerf-Bensussan N, Dautry-Varsat A (1992) Inhibition of clathrin- coated pit assembly by an EPS15 mutant. J Cell Sci 112:1303–1311
Damke H, Baba T, Warnock DE, Schmid SL (1994) Induction of mutant dynamin specifically blocks endocytic coated vesicle formation. J Cell Biol 127:915–934
Li G, Stahl PD (1993) Structure-function relationship of the small GTPase rab5. J Biol Chem 15:24475–24480
Negre D, Mangeot PE, Duisit G, Blanchard S, Vidalain PO, Leissner P, Winter AJ, Rabourdin-Combe C, Mehtali M, Moullier P, Darlix JL, Cosset FL (2000) Characterization of novel safe lentiviral vectors derived from simian immunodeficiency virus (SIVmac251) that efficiently transduce mature human dendritic cells. Gene Ther 7:1613–1623
Robertson MJ, Deane FM, Stahlschmidt W, von Kleist L, Haucke V, Robinson PJ, McCluskey A (2014) Synthesis of the Pitstop family of clathrin inhibitors. Nat Protoc 9:1592–1606
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Martínez, M.G., Forlenza, M.B., Candurra, N.A., Cordo, S.M. (2018). Entry Studies of New World Arenaviruses. In: Salvato, M. (eds) Hemorrhagic Fever Viruses. Methods in Molecular Biology, vol 1604. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6981-4_8
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DOI: https://doi.org/10.1007/978-1-4939-6981-4_8
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