Assaying Spontaneous Network Activity and Cellular Viability Using Multi-well Microelectrode Arrays
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Microelectrode array (MEA) technology is a neurophysiological method that allows for the spontaneous measure of activity in neural cultures and determination of drug and chemical effects thereon. Recent introduction of multi-well MEA (mwMEA) formats have dramatically increased the throughput of this technology, allowing more efficient compound screening. Rapid characterization of compounds for neuroactivity or neurotoxicity hazard evaluation following acute, chronic, or developmental exposures ideally would also consider compound effects on cell health, and to do so in the same well requires a multiplexed approach. Procedures describing the multiplexed method to acute and developmental screening are described in this chapter.
Key wordsMicroelectrode array (MEA) Neurophysiological method mwMEA Developmental screening
Preparation of this document has been funded by the U.S. Environmental Protection Agency. This document has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.
Jasmine P Brown is supported by Student Services Contracts EP-14-D-000101. Brittany S Lynch is an Oak Ridge Institute for Science and Education Fellow.
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