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Assaying Spontaneous Network Activity and Cellular Viability Using Multi-well Microelectrode Arrays

  • Jasmine P. Brown
  • Brittany S. Lynch
  • Itaevia M. Curry-Chisolm
  • Timothy J. Shafer
  • Jenna D. Strickland
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1601)

Abstract

Microelectrode array (MEA) technology is a neurophysiological method that allows for the spontaneous measure of activity in neural cultures and determination of drug and chemical effects thereon. Recent introduction of multi-well MEA (mwMEA) formats have dramatically increased the throughput of this technology, allowing more efficient compound screening. Rapid characterization of compounds for neuroactivity or neurotoxicity hazard evaluation following acute, chronic, or developmental exposures ideally would also consider compound effects on cell health, and to do so in the same well requires a multiplexed approach. Procedures describing the multiplexed method to acute and developmental screening are described in this chapter.

Key words

Microelectrode array (MEA) Neurophysiological method mwMEA Developmental screening 

Notes

Acknowledgments

Preparation of this document has been funded by the U.S. Environmental Protection Agency. This document has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.

Jasmine P Brown is supported by Student Services Contracts EP-14-D-000101. Brittany S Lynch is an Oak Ridge Institute for Science and Education Fellow.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Jasmine P. Brown
    • 1
  • Brittany S. Lynch
    • 1
  • Itaevia M. Curry-Chisolm
    • 1
  • Timothy J. Shafer
    • 1
  • Jenna D. Strickland
    • 2
    • 3
  1. 1.Integrated Systems Toxicology DivisionNHEERL, US EPA, MD105-05Research Triangle ParkUSA
  2. 2.Axion BiosystemsAtlantaUSA
  3. 3.Department of Pharmacology and ToxicologyMichigan State UniversityE. LansingUSA

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