Microbial Toxins pp 61-68

Part of the Methods in Molecular Biology book series (MIMB, volume 1600) | Cite as

Aptamer-Based Trapping: Enrichment of Bacillus cereus Spores for Real-Time PCR Detection

Protocol

Abstract

Aptamer-based trapping techniques are in general suitable to replace common antibody-based enrichment approaches. A time-consuming isolation or clean-up is often necessary during sample preparation, e.g. for the detection of spores. For the development of bioanalytical routine approaches, aptamers with a high affinity to B. cereus spores were applied for the establishment and validation of an aptamer-based trapping technique in milk with fat contents between 0.3 and 3.5%. Thereby, enrichment factors of up to sixfold were achieved. The combination of an aptamer-based enrichment by magnetic separation and the subsequent specific real-time PCR detection represents a reliable and rapid detection system.

Key words

Aptamer Bacillus cereus Spore trapping Real-time PCR Milk Food poisoning 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Hamburg School of Food Science, Institute of Food ChemistryUniversity of HamburgHamburgGermany

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