Aptamer-Based Trapping: Enrichment of Bacillus cereus Spores for Real-Time PCR Detection
Aptamer-based trapping techniques are in general suitable to replace common antibody-based enrichment approaches. A time-consuming isolation or clean-up is often necessary during sample preparation, e.g. for the detection of spores. For the development of bioanalytical routine approaches, aptamers with a high affinity to B. cereus spores were applied for the establishment and validation of an aptamer-based trapping technique in milk with fat contents between 0.3 and 3.5%. Thereby, enrichment factors of up to sixfold were achieved. The combination of an aptamer-based enrichment by magnetic separation and the subsequent specific real-time PCR detection represents a reliable and rapid detection system.
Key wordsAptamer Bacillus cereus Spore trapping Real-time PCR Milk Food poisoning
This research project was supported by the German Ministry of Economics and Technology (via AiF) and the FEI (Forschungskreis der Ernaehrungsindustrie e. V., Bonn, Germany); Project AiF 331 ZN.
The author’s thank Dr. Tim Hünniger, Jan-Hinnerk Jarck, and Esther Frohnmeyer for practical support and discussion.
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