A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis
Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.
Key wordsAB toxin Ricin Shiga toxin Toxin detection Toxin inhibitors Toxicity assay Vero cells
This material is based upon work supported by the US Department of Agriculture, Agricultural Research Service, under the Non-Assistance Cooperative Agreement (NACA) No. 58-5325-4-024. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the US Department of Agriculture. The authors thank Dr. Lucia Cilenti for technical assistance with the Accuri flow cytometer (BD Biosciences, San Jose, CA).