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A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis

  • Patrick Cherubin
  • Beatriz Quiñones
  • Salem Elkahoui
  • Wallace Yokoyama
  • Ken TeterEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1600)

Abstract

Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.

Key words

AB toxin Ricin Shiga toxin Toxin detection Toxin inhibitors Toxicity assay Vero cells 

Notes

Acknowledgments

This material is based upon work supported by the US Department of Agriculture, Agricultural Research Service, under the Non-Assistance Cooperative Agreement (NACA) No. 58-5325-4-024. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the US Department of Agriculture. The authors thank Dr. Lucia Cilenti for technical assistance with the Accuri flow cytometer (BD Biosciences, San Jose, CA).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Patrick Cherubin
    • 1
  • Beatriz Quiñones
    • 2
  • Salem Elkahoui
    • 3
  • Wallace Yokoyama
    • 4
  • Ken Teter
    • 1
    Email author
  1. 1.Burnett School of Biomedical Sciences, College of MedicineUniversity of Central FloridaOrlandoUSA
  2. 2.USDA-ARS, Produce Safety and Microbiology Research UnitWestern Regional Research CenterAlbanyUSA
  3. 3.Laboratoire des Substances Bioactives, Le Centre de Biotechnologie à la Technopole de Borj-CédriaHammam-LifTunisia
  4. 4.USDA-ARS, Healthy Processed Foods Research UnitWestern Regional Research CenterAlbanyUSA

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