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Biofluid Proteomics and Biomarkers in Traumatic Brain Injury

  • Safa Azar
  • Anwarul Hasan
  • Richard Younes
  • Farah Najdi
  • Lama Baki
  • Hussein Ghazale
  • Firas H. Kobeissy
  • Kazem Zibara
  • Stefania Mondello
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1598)

Abstract

Traumatic brain injury (TBI) is an injury to the brain caused by an external mechanical force, affecting millions of people worldwide. The disease course and prognosis are often unpredictable, and it can be challenging to determine an early diagnosis in case of mild injury as well as to accurately phenotype the injury. There is currently no cure for TBI—drugs having failed repeatedly in clinical trials—but an intense effort has been put to identify effective neuroprotective treatment. The detection of novel biomarkers, to understand more of the disease mechanism, facilitates early diagnosis, predicts disease progression, and develops molecularly targeted therapies that would be of high clinical interest. Over the last decade, there has been an increasing effort and initiative toward finding TBI-specific biomarker candidates. One promising strategy has been to use state-of-the-art neuroproteomics approaches to assess clinical biofluids and compare the cerebrospinal fluid (CSF) and blood proteome between TBI and control patients or between different subgroups of TBI. In this chapter, we summarize and discuss the status of biofluid proteomics in TBI, with a particular focus on the latest findings.

Key words

Proteomics Neuroproteomics Biomarkers TBI Brain injury Blood CSF Diagnosis Monitoring Outcome Clinical practice 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Safa Azar
    • 1
  • Anwarul Hasan
    • 2
    • 3
    • 4
    • 5
  • Richard Younes
    • 1
  • Farah Najdi
    • 1
  • Lama Baki
    • 1
  • Hussein Ghazale
    • 1
  • Firas H. Kobeissy
    • 6
    • 7
  • Kazem Zibara
    • 1
  • Stefania Mondello
    • 8
  1. 1.Department of Biochemistry and Molecular GeneticsAmerican University of BeirutBeirutLebanon
  2. 2.Department of Mechanical and Industrial EngineeringQatar UniversityDohaQatar
  3. 3.Biomedical Engineering and Department of Mechanical EngineeringAmerican University of BeirutBeirutLebanon
  4. 4.Center for Biomedical Engineering, Department of Medicine, Brigham and Women’s HospitalHarvard Medical SchoolCambridgeUSA
  5. 5.Harvard-MIT Division of Health Sciences and TechnologyMassachusetts Institute of TechnologyCambridgeUSA
  6. 6.Department of Biochemistry and Molecular Genetics, Faculty of MedicineAmerican University of BeirutBeirutLebanon
  7. 7.Department of Psychiatry, Center for Neuroproteomics and Biomarkers ResearchUniversity of FloridaGainesvilleUSA
  8. 8.Department of Biomedical, Odontoiatric and Morphological and Functional Imaging SciencesUniversity of Messina, A.O.U. “Policlinico G. Martino”MessinaItaly

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