Neuroproteomic Profiling of Cerebrospinal Fluid (CSF) by Multiplexed Affinity Arrays

  • Anna Häggmark-Månberg
  • Peter Nilsson
  • Jochen M. SchwenkEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1598)


Protein profiling through affinity proteomic approaches represents a powerful strategy for the analysis of human body fluids. Cerebrospinal fluid (CSF), being the fluid proximal to the central nervous system, is commonly analyzed in the context of neurological diseases, and can offer novel insights into the physiological state of the brain. Ultimately, and by analyzing the presence of brain-derived proteins in larger sets of samples that represent different phenotypes, profiling of CSF may serve as an important source to discover and verify disease-associated markers. Here, we describe a multiplexed and flexible protein profiling approach using antibody-based assays on suspension bead arrays. Through streamlined sample processing, protein biotinylation, and single-binder assay readout, this method enables high-throughput neuroproteomic analysis of up to 384 proteins in 384 samples.

Key words

Suspension bead array Antibody Microarray Cerebrospinal fluid Direct labeling Protein profiling 



We like to thank the entire staff of the Human Protein Atlas (HPA) for their efforts. This work was funded by the PRONOVA project (VINNOVA, Swedish Governmental Agency for Innovation Systems), and by grants from the Knut and Alice Wallenberg Foundation and Science for Life Laboratory, Stockholm.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Anna Häggmark-Månberg
    • 1
  • Peter Nilsson
    • 1
  • Jochen M. Schwenk
    • 1
    Email author
  1. 1.Affinity Proteomics, Science for Life Laboratory, School of BiotechnologyKTH - Royal Institute of TechnologySolnaSweden

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