Construction of Protein Switches by Domain Insertion and Directed Evolution
A protein switch is a protein that changes between inactive (“off”) and active (“on”) states in response to a biomolecule or physical signal. These switches can be created by fusing two domains in such a way that the activity of the output domain is regulated by the input domain’s recognition of an input signal (such as the binding of a molecule, recognition of light). Here, we describe several methods for randomly fusing two domains to create domain insertion libraries from which protein switches can be identified by selections and/or screens.
Key wordsProtein switch Domain insertion Circular permutation Directed evolution
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