Abstract
The liver is the largest internal organ and filters around 3 pints of blood per minute. This continuous flux of blood should not be confused with rapid egress of lymphocytes through the liver; this organ has intricate corridors of specialized sinusoidal spaces, ensuring that immune cells decelerate to shear flow rates, and providing ample opportunities to interact with parenchymal cells. Migration has been intricately linked to T cell function; it is therefore important to study liver T cell biology into context within the liver microenvironment. Here we discuss the highly organized architecture of liver-resident cells (sinusoidal endothelia, Kupffer cells, stellate cells/myofibroblasts, and biliary and hepatic epithelia) and showcase basic, multicellular, and complex systems to model T cell migration through the human liver microenvironment in vitro and ex vivo.
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Acknowledgements
We are grateful to our patient donors, staff, and volunteers at the Queen Elizabeth Hospital NHS Foundation Trust, and staff at the Centre for Liver Research at the University of Birmingham NIHR BRU for Liver Disease for their generous support. This work was supported by a Royal Society Dorothy Hodgkin Fellowship to Z.S. B.W. was funded by a MRC 4-year PhD studentship to Z.S. and K.A. was funded by a Dr. Falk Pharma studentship to GH. This work was supported by facilities funded by the National Institute for Health Research (NIHR) Birmingham Liver Biomedical Research Unit (BRU).
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Wiggins, B.G. et al. (2017). In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma. In: Rainger, G., Mcgettrick, H. (eds) T-Cell Trafficking. Methods in Molecular Biology, vol 1591. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6931-9_14
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DOI: https://doi.org/10.1007/978-1-4939-6931-9_14
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