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Cryobanking Mesenchymal Stem Cells

  • Andrés Pavón
  • Izaskun Beloqui
  • Juan M. Salcedo
  • Angel G. Martin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1590)

Abstract

Cryopreservation and storage of culture-expanded mesenchymal stem cells (MSCs) is essential for a biobank to maintain a collection of cell lines for research and clinical use. Optimization of cryopreservation protocols and methods to minimize damage to cells during freezing and thawing is critical to ensure reliable availability of viable cells. Controlling the freezing rate and the use of appropriate cryoprotectant, as well as stable storage temperature, can minimize the negative effects on cell viability. In this chapter, protocols for cryopreserving MSCs are described.

Key words

Cryopreservation Cryoprotectant DMSO Viability Control rate freezing Mesenchymal stem cells 

References

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    Nicol A, Nieda M, Donaldson C et al (1996) Cryopreserved human bone marrow stroma is fully functional in vitro. Br J Haematol 94:258–265CrossRefPubMedGoogle Scholar
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    Haack-Sorensen M, Bindslev L, Mortensen S et al (2007) The influence of freezing and storage on the characteristics and functions of human mesenchymal stromal cells isolated for clinical use. Cytotherapy 9:328–337CrossRefPubMedGoogle Scholar
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    Ware CB, Baran SW (2007) A controlled-cooling protocol for cryopreservation of human and non-human primate embryonic stem cells. Methods Mol Biol 407:43–49CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Andrés Pavón
    • 1
  • Izaskun Beloqui
    • 1
  • Juan M. Salcedo
    • 1
  • Angel G. Martin
    • 1
  1. 1.StemTek TherapeuticsDerioSpain

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