Cryobanking Pluripotent Stem Cells

  • Jeremy M. Crook
  • Eva Tomaskovic-Crook
  • Tenneille E. Ludwig
Part of the Methods in Molecular Biology book series (MIMB, volume 1590)


Cryobanking human pluripotent stem cells (hPSCs), be they human embryonic (hESCs) or induced pluripotent stem cells (iPSCs), is essential for their use in research and cell-based therapeutics. Working and master cell banks can be generated with a desired level of quality assurance applied during cell freezing and storage. Conventional vitrification has evolved to more advanced control rate freezing, culminating in a myriad of published protocols with variable proficiencies and clinical efficacies. Notwithstanding, standardized and reliable protocols are necessary for basic science through to applied research and clinical product development. This chapter details several methods for hPSC cryopreservation, suitable for routine application, high-quality research, and adaptable for clinical compliance.

Key words

Human pluripotent stem cells Vitrification Control rate freezing Cryopreservation Banking 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Jeremy M. Crook
    • 1
    • 2
    • 3
  • Eva Tomaskovic-Crook
    • 1
    • 2
  • Tenneille E. Ludwig
    • 4
  1. 1.ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research InstituteAIIM Facility, Innovation Campus, University of WollongongFairy MeadowAustralia
  2. 2.Illawarra Health and Medical Research InstituteUniversity of WollongongWollongongAustralia
  3. 3.Department of Surgery, St Vincent’s HospitalThe University of MelbourneFitzroyAustralia
  4. 4.WiCell Research InstituteMadisonUSA

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