Abstract
The phrase “oxidative-stress induced DNA damage” is commonly used in both the scientific literature and common media outlets, and is frequently linked to detrimental elements of aging as well as the onset of illnesses. Due to the growing focus on this topic, a clear need has emerged to develop a quantitative, low-cost methodology to allow for periodic monitoring of oxidative-stress induced DNA damage within individuals. Recent literature examining the link between oxidative stress and the onset of various cancers has made monitoring an even more pressing need. The mechanism of oxidative-stress induced DNA damage originates in chronic inflammation, which in turn activates various transcription factors and diseases that influence the onset of tumor development, chemoresistance, radioresistance, and other harmful cellular processes. While current technologies that aim to provide quantitative metrics require extremely expensive equipment and significant technical expertise, our laboratory has designed a low-cost methodology utilizing a combination of carbon nanotubes, paper electrodes, and immunochromatographic strips.
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Acknowledgment
This research work is partially supported under grants NIH R15 ES021079-01, NSF 1334417 and Shanxi Hundred-Talent Program.
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Leichner, J. et al. (2017). Electrochemical Lateral Flow Paper Strip for Oxidative-Stress Induced DNA Damage Assessment. In: Prickril, B., Rasooly, A. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1572. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6911-1_3
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DOI: https://doi.org/10.1007/978-1-4939-6911-1_3
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