Extended Gate Field-Effect Transistor Biosensors for Point-Of-Care Testing of Uric Acid

  • Weihua GuanEmail author
  • Mark A. Reed
Part of the Methods in Molecular Biology book series (MIMB, volume 1572)


An enzyme-free redox potential sensor using off-chip extended-gate field effect transistor (EGFET) with a ferrocenyl-alkanethiol modified gold electrode has been used to quantify uric acid concentration in human serum and urine. Hexacyanoferrate (II) and (III) ions are used as redox reagent. The potentiometric sensor measures the interface potential on the ferrocene immobilized gold electrode, which is modulated by the redox reaction between uric acid and hexacyanoferrate ions. The device shows a near Nernstian response to uric acid and is highly specific to uric acid in human serum and urine. The interference that comes from glucose, bilirubin, ascorbic acid, and hemoglobin is negligible in the normal concentration range of these interferents. The sensor also exhibits excellent long term reliability and is regenerative. This extended gate field effect transistor based sensor is promising for point-of-care detection of uric acid due to the small size, low cost, and low sample volume consumption.

Key words

Extended gate FET Redox Enzyme-free Uric acid Potentiometric sensor Ferrocenyl-alkanethiol 



W.G. acknowledges the financial support from Howard Hughes Medical Institute International Student Research Fellowship and Pennsylvania State University. The work was supported in part by the Defense Threat Reduction Agency under grants HDTRA1-10-1-0037 and HDTRA-1-12-1-0042, and by the U. S. Army Research Laboratory and the U. S. Army Research Office under contract/grant number MURI W911NF-11-1-0024.


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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Electrical EngineeringPennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of Electrical EngineeringYale UniversityNew HavenUSA
  3. 3.Applied PhysicsYale UniversityNew HavenUSA

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