Protein-Carbohydrate Interactions pp 255-277

Part of the Methods in Molecular Biology book series (MIMB, volume 1588)

Metagenomics and CAZyme Discovery

  • Benoit J. Kunath
  • Andreas Bremges
  • Aaron Weimann
  • Alice C. McHardy
  • Phillip B. Pope
Protocol

Abstract

Microorganisms play a primary role in regulating biogeochemical cycles and are a valuable source of enzymes that have biotechnological applications, such as carbohydrate-active enzymes (CAZymes). However, the inability to culture the majority of microorganisms that exist in natural ecosystems using common culture-dependent techniques restricts access to potentially novel cellulolytic bacteria and beneficial enzymes. The development of molecular-based culture-independent methods such as metagenomics enables researchers to study microbial communities directly from environmental samples, and presents a platform from which enzymes of interest can be sourced. We outline key methodological stages that are required as well as describe specific protocols that are currently used for metagenomic projects dedicated to CAZyme discovery.

Key words

Metagenomics Carbohydrate active enzymes Microbial communities Assembly Binning 

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Benoit J. Kunath
    • 1
  • Andreas Bremges
    • 2
    • 3
  • Aaron Weimann
    • 2
  • Alice C. McHardy
    • 2
  • Phillip B. Pope
    • 1
  1. 1.Department of Chemistry, Biotechnology and Food ScienceNorwegian University of Life SciencesÅsNorway
  2. 2.Computational Biology of Infection ResearchHelmholtz Centre for Infection ResearchBraunschweigGermany
  3. 3.German Center for Infection Research (DZIF)BraunschweigGermany

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