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Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds

  • Clement Lin
  • Danzhou YangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1587)

Abstract

G-quadruplexes are noncanonical secondary structures formed in DNA sequences containing consecutive runs of guanines. It has been shown that the 3′ G-rich single-stranded overhangs of human telomeres can form G-quadruplex structures, and the human telomeric DNA G-quadruplexes are considered attractive targets for anticancer drugs. G-quadruplex-interactive compounds have been shown to inhibit telomerase access as well as telomere capping. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method in determining the G-quadruplex structures under physiologically relevant conditions. We present the NMR and biophysical methodology used in our research group for the study of G-quadruplex structures in physiologically relevant solution and their interactions with small-molecule compounds.

Key words

Human telomeres G-Quadruplex structures Structure polymorphism G-Quadruplex-interactive compounds Telomerase inhibitors Anticancer drug targets 

Notes

Acknowledgments

This research was supported by the National Institutes of Health funding (1S10RR16659, CA122952, GM083117, and CA177585). We thank Dr. Megan Carver for proofreading the manuscript.

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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Medicinal Chemistry and Molecular Pharmacology, College of PharmacyPurdue UniversityWest LafayetteUSA
  2. 2.Purdue Center for Cancer ResearchPurdue UniversityWest LafayetteUSA
  3. 3.Purdue Institute for Drug DiscoveryPurdue UniversityWest LafayetteUSA

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