The Immune Synapse: Past, Present, and Future
Immunological synapses are specialized cell-cell junctions characterized by (1) close apposition of the immune cell membrane with the membrane of another cell driven by adaptive or innate immune recognition, (2) adhesion, (3) stability, and (4) directed secretion. This phenomenon was first recognized in the 1970s and the early 1980s through electron microscopy of ex vivo functioning immune cells. Progressive advances in fluorescence microscopy and molecular immunology in the past 20 years have led to rapid progress on understanding the modes of cell-cell interaction and underlying molecular events. This volume contains a diverse range of protocols that can be applied to the study of the immunological synapses and related immune cell junctions both in vitro and in vivo; and in disease settings in animal models and humans. We have also included chapters on critical molecular tools such as protein expression and mRNA electroporation that underpin or expand imaging approaches, although they are not specific to the study of immune synapses. We hope that these chapters will be of use to people entering the field as well as seasoned practitioners looking to expand their repertoire of methods.
Key wordsScience history Fluorescence Affinity Modeling Microscopy
We thank Éva Culleton-Oltay for assistance with editing the chapters and all the authors for their hard work on this project.
- 9.Norcross MA (1984) A synaptic basis for T-lymphocyte activation. Ann Immunol 135D(2):113–134Google Scholar
- 17.Carroll-Portillo A, Spendier K, Pfeiffer J, Griffiths G, Li H, Lidke KA, Oliver JM, Lidke DS, Thomas JL, Wilson BS, Timlin JA (2010) Formation of a mast cell synapse: Fc epsilon RI membrane dynamics upon binding mobile or immobilized ligands on surfaces. J Immunol 184(3):1328–1338CrossRefPubMedGoogle Scholar
- 18.Goodridge HS, Reyes CN, Becker CA, Katsumoto TR, Ma J, Wolf AJ, Bose N, Chan AS, Magee AS, Danielson ME, Weiss A, Vasilakos JP, Underhill DM (2011) Activation of the innate immune receptor Dectin-1 upon formation of a 'phagocytic synapse'. Nature 472(7344):471–475CrossRefPubMedPubMedCentralGoogle Scholar
- 19.Malinova D, Fritzsche M, Nowosad CR, Armer H, Munro PM, Blundell MP, Charras G, Tolar P, Bouma G, Thrasher AJ (2015) WASp-dependent actin cytoskeleton stability at the dendritic cell immunological synapse is required for extensive, functional T cell contacts. J Leukoc Biol. doi: 10.1189/jlb.2A0215-050RR PubMedGoogle Scholar
- 28.Schubert DA, Gordo S, Sabatino JJ Jr, Vardhana S, Gagnon E, Sethi DK, Seth NP, Choudhuri K, Reijonen H, Nepom GT, Evavold BD, Dustin ML, Wucherpfennig KW (2012) Self-reactive human CD4 T cell clones form unusual immunological synapses. J Exp Med 209(2):335–352CrossRefPubMedPubMedCentralGoogle Scholar