Protocol

Recombinant Virus Vaccines

Volume 1581 of the series Methods in Molecular Biology pp 203-222

Date:

Generating Recombinant Vesicular Stomatitis Viruses for Use as Vaccine Platforms

  • John B. RuedasAffiliated withDepartment of Microbiology and National Emerging Infectious Disease Laboratory, Boston University School of Medicine
  • , John H. ConnorAffiliated withDepartment of Microbiology and National Emerging Infectious Disease Laboratory, Boston University School of Medicine Email author 

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Abstract

The unique properties of vesicular stomatitis virus (VSV) make it a promising vaccine platform. With the advent of plasmid-based approaches to generate recombinant VSV viruses that express glycoproteins of other viruses, researchers now have the means to generate vaccine candidates targeting a variety of human pathogens. This chapter gives a general overview of the workings of VSV as a vaccine platform and provides a detailed protocol for the generation of recombinant VSV from plasmids.

Key words

Vesicular stomatitis virus Vaccine platform Reverse genetics Vaccinia virus T7 RNA polymerase