Skip to main content

Surface Acoustic Wave Lysis and Ion-Exchange Membrane Quantification of Exosomal MicroRNA

Part of the Methods in Molecular Biology book series (MIMB,volume 1580)


MicroRNA detection and quantification are commonly explored techniques for diagnostic and prognostic predictions. Typically, microRNAs are extracted and purified from a biological source, converted into complementary DNA (cDNA), and amplified using real time polymerase chain reaction (RT-PCR). The number of RT-PCR cycles required to reach the threshold of detection provides a relative quantification of the target microRNA when this data is normalized to the quantity of a control microRNA. This methodology has several drawbacks, including the need to artificially amplify the target microRNA for detection as well as quantification errors that can occur due to expression level differences of the control microRNAs for normalization in various sample sources. Here, we provide a technique to quantify actual concentrations of target microRNAs directly from any biological source without the requirement of these additional steps. In addition, we describe an alternative approach for obtaining exosomal microRNAs directly from biological samples without the use of harsh detergents and RNA isolation.

Key words

  • MicroRNA
  • RNA
  • Detection
  • Quantification
  • Exosomes
  • SAW
  • Microfluidics
  • Ion-exchange
  • Membrane
  • Biosensor

This is a preview of subscription content, access via your institution.

Buying options

USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-1-4939-6866-4_5
  • Chapter length: 12 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
USD   139.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6866-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   179.99
Price excludes VAT (USA)
Hardcover Book
USD   179.99
Price excludes VAT (USA)
Fig. 1
Fig. 2
Fig. 3
Fig. 4

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more


  1. Taller D, Richards K, Slouka Z et al (2015) On-Chip surface acoustic wave lysis and ion-exchange nanomembrane detection of exosomal RNA for pancreatic cancer study and diagnosis. Lab Chip 15:1656–1666

    CAS  CrossRef  PubMed  Google Scholar 

  2. Senapati S, Slouka Z, Shah S et al (2014) An ion-exchange nanomembrane sensor for detection of nucleic acids using a surface charge inversion phenomenon. Biosens Bioelectron 60:92–100

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  3. Slouka Z, Senapati S, Chang H (2014) Microfluidic systems with ion-selective membranes. Annu Rev Anal Chem 7:317–335

    CAS  CrossRef  Google Scholar 

  4. Gallo A, Tandon M, Alevizos I, Illei GG (2012) The majority of MicroRNAs detectable in serum and saliva is concentrated in exosomes. PLoS One 7(3):e30679

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  5. Länge K, Rapp B, Rapp M (2008) Surface acoustic wave biosensors: a review. Anal Bioanal Chem 391:1509–1519

    CrossRef  PubMed  Google Scholar 

  6. Taller D, Go D, Chang H (2013) Modulated exponential films generated by surface acoustic waves and their role in liquid wicking and aerosolization at a pinned drop. Physical Review E 87:053004

    CrossRef  Google Scholar 

  7. Hartman CS, Abbott BP (1989) Overview of design challenges for single phase unidirectional SAW filters. Ultrasonics Symposium IEEE 1: 79–89

    Google Scholar 

  8. Shilton R, Tan M, Yeo L et al (2008) Particle concentration and mixing in microdrops driven by focused acoustic waves. J Appl Phys 104:014910

    CrossRef  Google Scholar 

  9. Qi A, Yeo L, Friend J et al (2009) The extraction of liquid, protein molecules and yeast cells from paper through surface acoustic wave atomization. Lab Chip 10:470–476

    CrossRef  PubMed  Google Scholar 

  10. Taller D (2015) Surface acoustic wave microfluidics: droplets, pinned liquid films, and biodetection. Dissertation, University of Notre Dame

    Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Reginald Hill .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Richards, K.E., Go, D.B., Hill, R. (2017). Surface Acoustic Wave Lysis and Ion-Exchange Membrane Quantification of Exosomal MicroRNA. In: Dalmay, T. (eds) MicroRNA Detection and Target Identification. Methods in Molecular Biology, vol 1580. Humana Press, New York, NY.

Download citation

  • DOI:

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6864-0

  • Online ISBN: 978-1-4939-6866-4

  • eBook Packages: Springer Protocols