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Monitoring of Crosstalk Between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots

  • Víctor Carrasco Loba
  • Marta-Marina Pérez Alonso
  • Stephan PollmannEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1569)

Abstract

Over the last few years, it became more and more evident that plant hormone action is to great parts determined through their sophisticated crosstalk, rather than by their isolated activities. Thus, the parallel analysis of interconnected phytohormones in only very small amounts of tissue developed to an important issue in the field of plant sciences. In the following, a highly sensitive and accurate method is described for the quantitative analysis of the plant hormones jasmonic acid and indole-3-acetic acid in the model plant Arabidopsis thaliana. The described methodology is, however, not limited to the analysis of Arabidopsis samples but can also be applied to other plant species. The presented method is optimized for the working up of as little as 20–50 mg of plant tissue. Thus, it is well suited for the analysis of plant hormone contents in plant tissue of only little biomass, such as roots. The presented protocol facilitates the implementation of the method into other laboratories that have access to appropriate laboratory equipment and comparable state-of-the-art gas chromatography-mass spectrometry (GC-MS) technology.

Key words

Jasmonic acid Auxin Crosstalk Electron impact tandem mass spectrometry (EI-GC-MS/MS) Solid-phase extraction Stable isotopes Derivatization Plant hormone analysis 

Notes

Acknowledgments

This work was supported by MINECO grant BFU2014-55575-R and Marie Curie career integration grant FP7-PEOPLE-CIG-2011 303744 to SP.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Víctor Carrasco Loba
    • 1
  • Marta-Marina Pérez Alonso
    • 1
  • Stephan Pollmann
    • 1
    Email author
  1. 1.Centro de Biotecnología y Genómica de PlantasUniversidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA)Pozuelo de Alarcón, MadridSpain

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