CD95 pp 95-102

Part of the Methods in Molecular Biology book series (MIMB, volume 1557)

CD95-Mediated Proton Regulation

  • Auréa Cophignon
  • Mallorie Poët
  • Michael Monet
  • Michel Tauc
  • Laurent Counillon
Protocol
First Online:

Abstract

The Na+/H+ exchanger NHE1 is at the crossroads of a large diversity of signaling pathways, whose activation modifies the cooperative response of the transporter to intracellular H+ ions. Here we show how the activation of the Na+/H+ exchanger NHE1 by the cleaved ligand of CD95 can be measured. We demonstrate two different methods designed to set intracellular pH at precise values. Then we show how these can be coupled to fast kinetics of lithium transport, which will enable to measure the NHE1 activity like for an enzyme, because they will yield rates of transport.

Key words

Ion transport Kinetic measurements Intracellular acidification CD95 signaling 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Auréa Cophignon
    • 1
    • 2
  • Mallorie Poët
    • 1
    • 2
  • Michael Monet
    • 1
    • 2
    • 3
  • Michel Tauc
    • 1
    • 2
  • Laurent Counillon
    • 1
    • 2
  1. 1.LP2M UMR 7370 Faculté de MédecineUniversité Nice-Sophia AntipolisNiceFrance
  2. 2.Laboratories of Excellence, Ion Channels Sciences and TherapeuticsUniversité LilleVilleneuve d’AscqFrance
  3. 3.LPCM EA4667Université de Picardie Jules VerneAmiensFrance

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