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Effects of Macrophage Conditioned-Medium on Murine and Human Muscle Cells: Analysis of Proliferation, Differentiation, and Fusion

  • Marielle Saclier
  • Marine Theret
  • Rémi Mounier
  • Bénédicte ChazaudEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1556)

Abstract

Skeletal muscle is a highly plastic tissue, which is able to regenerate after an injury. Effective and complete regeneration requires interactions between myogenic precursor cells and several cell types such as macrophages. Bone marrow derived macrophages in mouse and monocyte-derived macrophages in human are useful tools to obtain macrophage populations that may be specifically activated/polarized in vitro (e.g., pro-inflammatory, anti-inflammatory, and alternatively activated macrophages). In vitro, human or murine primary myogenic cells recapitulate the adult myogenesis program through proliferation, myogenic differentiation, and fusion. Macrophages being highly secreting cells, they act on various biological processes including adult myogenesis. Here, we present protocols to analyze in vitro the effect of macrophage-secreted factors on muscle cell proliferation or differentiation in both mouse and human.

Key words

Macrophages Muscle precursor cells Proliferation Differentiation Myogenesis Human Mouse Conditioned media 

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Copyright information

© Springer Science+Business Media LLC 2017

Open Access This chapter is distributed under the terms of the Creative Commons Attribution Noncommercial License, which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Authors and Affiliations

  • Marielle Saclier
    • 1
  • Marine Theret
    • 2
    • 4
  • Rémi Mounier
    • 2
    • 3
  • Bénédicte Chazaud
    • 2
    Email author
  1. 1.Department of BiosciencesUniversity of MilanMilanItaly
  2. 2.Institut NeuroMyoGène, INMG, Université Claude Bernard Lyon 1VilleurbanneFrance
  3. 3.Université Claude Bernard Lyon 1VilleurbanneFrance
  4. 4.Université Paris Descartes, Sorbonne Paris CitéParisFrance

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