Protocols for Studying Inhibition and Eradication of Bacterial Biofilms by Antimicrobial Peptides

  • Vijayalekshmi Sarojini
Part of the Methods in Molecular Biology book series (MIMB, volume 1548)


Many pathogenic microorganisms have the ability to form biofilms that are impervious to conventional antibiotics making these pathogens resistant to multiple antibiotics. This necessitates the development of novel antimicrobial compounds with less chance of resistance development and the ability to penetrate the extracellular polymer matrix of bacterial biofilms. In this report, simple assays to test the antibiofilm potential of antimicrobial peptides are described.

Key words

Antimicrobial peptides Bacterial biofilms Antibiotic resistance Confocal microscopy Live/Dead stain Crystal violet stain 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.School of Chemical SciencesThe University of AucklandAucklandNew Zealand

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