Protocols for Studying the Interaction of MSI-78 with the Membranes of Whole Gram-Positive and Gram-Negative Bacteria by NMR

  • Nury P. Santisteban
  • Michael R. Morrow
  • Valerie Booth
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1548)

Abstract

Antimicrobial peptides (AMPs) may interact with a variety of target cell components, including the lipid bilayer, non-lipidic cell envelope components, and/or intracellular targets. However, most biophysical experiments aimed at elucidating the detailed mechanism of AMPs are limited to simple model membrane systems and neglect potentially functional interactions between AMPs and non-lipidic cell components. One of the biophysical techniques commonly used to study how AMPs interact with lipid bilayers is solid-state deuterium NMR. In this chapter we provide protocols to prepare deuterium-labeled intact Gram-negative and Gram-positive bacteria and to observe these samples using solid-state deuterium NMR. Such experiments have the potential to provide important information about how non-lipidic cell envelope components modulate AMP interactions with the cytoplasmic membrane of bacteria.

Key words

AMP Antimicrobial peptide Solid-state NMR Deuterium NMR Whole-cell NMR 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Nury P. Santisteban
    • 1
  • Michael R. Morrow
    • 1
  • Valerie Booth
    • 1
    • 2
  1. 1.Department of Physics and Physical OceanographyMemorial University of Newfoundland and LabradorSt. John’sCanada
  2. 2.Department of BiochemistryMemorial University of Newfoundland and LabradorSt. John’sCanada

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