Isolation of Platelet-Derived Extracellular Vesicles
Platelets participate in several physiological functions, including hemostasis, immunity, and development. Additionally, platelets play key roles in arterial thrombosis and cancer progression. Given this plethora of functions, there is a strong interest of the role of platelet-derived (extracellular) vesicles (PDEVs) as functional mediators and biomarkers. Moreover, the majority of the blood-borne EVs are thought to originate from either platelets or directly from the platelet precursor cells, the megakaryocytes, which reside in the bone marrow. To circumvent confusion, we use the term PDEVs for both platelet-derived and/or megakaryocyte-derived EVs. PDEVs can be isolated from blood or from isolated platelets after activation. In this chapter, we describe all commonly used PDEV isolation methods from blood and prepurified platelets.
Key wordsExtracellular vesicle Platelet Microparticle Exosome Isolation Size-exclusion chromatography Immunobeads Gradient centrifugation
Part of this work was funded by the European Metrology Research Programme (EMRP) under the Joint Research Project HLT02 (www.metves.eu). The EMRP is jointly funded by the EMRP participating countries within the European Association of National Metrology Institutes and the European Union (S.V., Y.Y., and R.N.).
Part of this work was funded by the Magnus Ehrnrooth Foundation, the Medicinska Understödsförening Liv och Hälsa r.f., the Otto Malm Foundation, and the Oscar Öflund Foundation (M.A. and P.S.). Part of the work was funded by the SalWe Research Program GET IT DONE Tekes grant nro 3986/31/2013 (S.V., P.S.).
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