Xylem pp 59-65 | Cite as

Tissue Culture for Xylem Differentiation with Arabidopsis Leaves

  • Masato Saito
  • Alif Meem Nurani
  • Yuki KondoEmail author
  • Hiroo FukudaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1544)


Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for xylem cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic xylem cells can be induced throughout leaf disks within 3–4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular cell induction culture.

Key words

Culture system Arabidopsis Transdifferentiation Reprogramming Mesophyll Procambium Xylem 



This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (15H01226 to Y.K., and 15H05958 and NC-CARP project to H.F.), from the Japan Society for the Promotion of Science (26891005 to Y.K. and 23227001 to H.F.) and from Naito Foundation to H.F.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Biological Sciences, Graduate School of ScienceThe University of TokyoTokyoJapan

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