Tissue Culture for Xylem Differentiation with Arabidopsis Leaves
Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for xylem cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic xylem cells can be induced throughout leaf disks within 3–4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular cell induction culture.
Key wordsCulture system Arabidopsis Transdifferentiation Reprogramming Mesophyll Procambium Xylem
This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (15H01226 to Y.K., and 15H05958 and NC-CARP project to H.F.), from the Japan Society for the Promotion of Science (26891005 to Y.K. and 23227001 to H.F.) and from Naito Foundation to H.F.