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Xylem pp 59-65 | Cite as

Tissue Culture for Xylem Differentiation with Arabidopsis Leaves

  • Masato Saito
  • Alif Meem Nurani
  • Yuki KondoEmail author
  • Hiroo FukudaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1544)

Abstract

Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for xylem cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic xylem cells can be induced throughout leaf disks within 3–4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular cell induction culture.

Key words

Culture system Arabidopsis Transdifferentiation Reprogramming Mesophyll Procambium Xylem 

Notes

Acknowledgment

This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (15H01226 to Y.K., and 15H05958 and NC-CARP project to H.F.), from the Japan Society for the Promotion of Science (26891005 to Y.K. and 23227001 to H.F.) and from Naito Foundation to H.F.

References

  1. 1.
    Fukuda H, Komamine A (1980) Establishment of an experimental system for the study of tracheary element differentiation from single cells isolated from the mesophyll of Zinnia elegans. Plant Physiol 65:57–60CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Kubo M, Udagawa M, Nishikubo N, Horiguchi G, Yamaguchi M, Ito J, Mimura T, Fukuda H, Demura T (2005) Transcription switches for protoxylem and metaxylem vessel formation. Genes Dev 19:1850–1860CrossRefGoogle Scholar
  3. 3.
    Oda Y, Iida Y, Kondo Y, Fukuda H (2010) Wood cell-wall structure requires local 2D-microtubule disassembly by a novel plasma membrane-anchored protein. Curr Biol 20:1197–1202CrossRefPubMedGoogle Scholar
  4. 4.
    Pesquet E, Korolev AV, Calder G, Lloyd CW (2010) The microtubule-associated protein AtMAP70-5 regulates secondary wall patterning in Arabidopsis wood cells. Curr Biol 20:744–749CrossRefPubMedGoogle Scholar
  5. 5.
    Kondo Y, Ito T, Nakagami H, Hirakawa Y, Saito M, Tamaki T, Shirasu K, Fukuda H (2014) Plant GSK3 proteins regulate xylem cell differentiation downstream of TDIF-TDR signalling. Nat Commun 5:3504PubMedGoogle Scholar
  6. 6.
    Kondo Y, Fukuda H (2015) The TDIF signaling network. Curr Opin Plant Biol 28:106–110CrossRefPubMedGoogle Scholar
  7. 7.
    Kondo Y, Fujita T, Sugiyama M, Fukuda H (2015) A novel system for xylem cell differentiation in Arabidopsis thaliana. Mol Plant 8:612–621CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Biological Sciences, Graduate School of ScienceThe University of TokyoTokyoJapan

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