Targeting Trichothecene Biosynthetic Genes

  • Songhong Wei
  • Theo van der Lee
  • Els Verstappen
  • Marga van Gent
  • Cees WaalwijkEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1542)


Biosynthesis of trichothecenes requires the involvement of at least 15 genes, most of which have been targeted for PCR. Qualitative PCRs are used to assign chemotypes to individual isolates, e.g., the capacity to produce type A and/or type B trichothecenes. Many regions in the core cluster (consisting of 12 genes) including intergenic regions have been used as targets for PCR, but the most robust assays are targeted to the tri3 and tri12 genes. Quantitative PCRs, that work across trichothecene-producing members of the Fusarium head blight complex, are described along with procedures to quantify the amount of fungal biomass in wheat samples. These assays are directed to the chemotype(s) present in field samples and quantify the total fungal biomass of trichothecene-producing fungi, irrespective of their genetic identity.

Key words

Trichothecenes PCR Quantitative PCR 



This work was supported by the MycoRed project (FP7 Food Quality and Safety Priority—Large Collaborative Project—GA 222690) and the Dutch Main Board for Arable Products.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Songhong Wei
    • 1
  • Theo van der Lee
    • 2
  • Els Verstappen
    • 2
  • Marga van Gent
    • 2
  • Cees Waalwijk
    • 2
    Email author
  1. 1.College of Plant ProtectionShenyang Agricultural UniversityShenyangPeople’s Republic of China
  2. 2.Biointeractions and Plant HealthWageningen URWageningenThe Netherlands

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