Epstein Barr Virus pp 255-265

Part of the Methods in Molecular Biology book series (MIMB, volume 1532)

| Cite as

EBV-Directed T Cell Therapeutics for EBV-Associated Lymphomas

  • Lauren P. McLaughlin
  • Stephen Gottschalk
  • Cliona M. Rooney
  • Catherine M. Bollard


Epstein Barr virus (EBV) is a human gamma herpes virus that establishes latency in B cells after primary infection. EBV generally only causes a mild, self-limiting viral illness but is also associated with several malignancies including posttransplantation lymphoproliferative disorder in the immunosuppressed host as well as Hodgkin and non-Hodgkin lymphoma in the immune competent host. The expression of EBV antigens by lymphoma has important applications as targets for adoptive T cell therapy. However, as many lymphomas only express subdominant EBV antigens that are less immunogenic, novel strategies are needed to manufacture EBV-specific T cell products specific for Latent Membrane Protein 1 (LMP1) and LMP2, which are expressed in lymphomas with type II and III latency. While several techniques for manufacturing EBV-CTLs are described in the literature, this chapter focuses on one method for generating Good Manufacturing Practice (GMP)-compliant EBV-specific T cell products that are enriched with LMP1 and LMP2.

Key words

Epstein Barr virus Lymphoma Adoptive T cell therapy Good manufacturing practice 


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Lauren P. McLaughlin
    • 1
    • 2
  • Stephen Gottschalk
    • 3
  • Cliona M. Rooney
    • 4
  • Catherine M. Bollard
    • 2
    • 5
  1. 1.Department of Hematology/OncologyChildren’s National Medical CenterWashington, DCUSA
  2. 2.The George Washington UniversityWashington, DCUSA
  3. 3.Texas Children’s Cancer Center, Texas Children’s HospitalBaylor College of MedicineHoustonUSA
  4. 4.Center for Cell and Gene Therapy, Texas Children’s Hospital, Houston Methodist Hospital,Baylor College of MedicineHoustonUSA
  5. 5.Center for Cancer and Immunology Research, Children’s National Medical CenterWashington, DCUSA

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