Protocol

Cancer Nanotechnology

Volume 1530 of the series Methods in Molecular Biology pp 247-254

Date:

Quantitative Evaluation of the Enhanced Permeability and Retention (EPR) Effect

  • Luisa M. RussellAffiliated withDepartment of Materials Science and Engineering, Johns Hopkins UniversityInstitute for NanoBioTechnology, Johns Hopkins University Email author 
  • , Charlene M. DawidczykAffiliated withDepartment of Materials Science and Engineering, Johns Hopkins UniversityInstitute for NanoBioTechnology, Johns Hopkins University
  • , Peter C. SearsonAffiliated withDepartment of Materials Science and Engineering, Johns Hopkins UniversityInstitute for NanoBioTechnology, Johns Hopkins UniversityDepartment of Oncology, Johns Hopkins University School of Medicine

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Abstract

Quantitative evaluation of nanoparticle delivery to a tumor site can be invaluable for cross-platform comparison, a consideration not currently taken into account by many in the field of cancer nanomedicine (Dawidczyk et al., Front Chem 2:69, 2014). Standardization of measured parameters and experimental design will facilitate nanoparticle design and understanding in the field. Here, we present a broadly applicable in vivo protocol for preclinical trials of nanomedicines, including pharmacokinetic modeling and recommendations for parameters to be reported for nanoparticle evaluation. The proposed protocol is simple and not prohibitively mouse-heavy, using procedures that are not overly complicated or difficult to learn, yet is a powerful way to analyze the effectiveness of new cancer nanomedicines against standard or more developed ones.

Key words

Tumor uptake Nanoparticles Enhanced permeability and retention (EPR) effect Drug delivery systems Pharmacokinetics