Bioinformatics pp 257-270 | Cite as

Studying Antibody Repertoires with Next-Generation Sequencing

  • William D. Lees
  • Adrian J. ShepherdEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1526)


Next-generation sequencing is making it possible to study the antibody repertoire of an organism in unprecedented detail, and, by so doing, to characterize its behavior in the response to infection and in pathological conditions such as autoimmunity and cancer. The polymorphic nature of the repertoire poses unique challenges that rule out the use of many commonly used NGS methods and require tradeoffs to be made when considering experimental design.

We outline the main contexts in which antibody repertoire analysis has been used, and summarize the key tools that are available. The humoral immune response to vaccination has been a particular focus of repertoire analyses, and we review the key conclusions and methods used in these studies.

Key words

Antibodies Antibodyome Repertoire analysis Rep-Seq Next generation sequencing 


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Biological Sciences and Institute of Structural and Molecular Biology, BirkbeckUniversity of LondonLondonUK

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