Liposomes pp 17-22 | Cite as

Thin-Film Hydration Followed by Extrusion Method for Liposome Preparation

  • Hongwei Zhang
Part of the Methods in Molecular Biology book series (MIMB, volume 1522)


One of the simplest ways to prepare liposomes in a research laboratory is the thin-film hydration method followed by extrusion. This method involves making a thin lipid film in a round-bottom flask by the removal of organic solvent. Upon the addition and agitation of the dispersion medium, heterogeneous liposomes are formed. Finally, after extrusion through polycarbonate membranes, homogeneous small liposomes are obtained.

Key words

Liposomes Thin-film hydration Extrusion Phase transition temperature Drug delivery 


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.School of Pharmacy – BostonMCPHS UniversityBostonUSA

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