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Liposomes pp 17-22 | Cite as

Thin-Film Hydration Followed by Extrusion Method for Liposome Preparation

  • Hongwei Zhang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1522)

Abstract

One of the simplest ways to prepare liposomes in a research laboratory is the thin-film hydration method followed by extrusion. This method involves making a thin lipid film in a round-bottom flask by the removal of organic solvent. Upon the addition and agitation of the dispersion medium, heterogeneous liposomes are formed. Finally, after extrusion through polycarbonate membranes, homogeneous small liposomes are obtained.

Key words

Liposomes Thin-film hydration Extrusion Phase transition temperature Drug delivery 

References

  1. 1.
    Bangham AD, Horne RW (1964) Negative staining of phospholipids and their structural modification by surface-active agents as observed in the electron microscope. J Mol Biol 8:660–668CrossRefPubMedGoogle Scholar
  2. 2.
    Sessa G, Weissmann G (1970) Incorporation of lysozyme into liposomes. A model for structure-linked latency. J Biol Chem 245:3295–3301PubMedGoogle Scholar
  3. 3.
    Gregoriadis G (1976) The carrier potential of liposomes in biology and medicine (first of two parts). N Engl J Med 295:704–710CrossRefPubMedGoogle Scholar
  4. 4.
    Gregoriadis G (1976) The carrier potential of liposomes in biology and medicine (second of two parts). N Engl J Med 295:765–770CrossRefPubMedGoogle Scholar
  5. 5.
    van Rooijen N (1990) Liposomes as carrier and immunoadjuvant of vaccine antigens. Adv Biotechnol Processes 13:255–279PubMedGoogle Scholar
  6. 6.
    Richards RL, Hayre MD, Hockmeyer WT, Alving CR (1988) Liposomes as carriers for a human malaria sporozoite vaccine. Biochem Soc Trans 16:921–922CrossRefPubMedGoogle Scholar
  7. 7.
    Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M, Northrop JP, Ringold GM, Danielsen M (1987) Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci U S A 84:7413–7417CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Silindir M, Erdogan S, Ozer AY, Maia S (2012) Liposomes and their applications in molecular imaging. J Drug Target 20:401–415CrossRefPubMedGoogle Scholar
  9. 9.
    Weiner N, Lieb L, Niemiec S, Ramachandran C, Hu Z, Egbaria K (1994) Liposomes: a novel topical delivery system for pharmaceutical and cosmetic applications. J Drug Target 2:405–410CrossRefPubMedGoogle Scholar
  10. 10.
    Betz G, Aeppli A, Menshutina N, Leuenberger H (2005) In vivo comparison of various liposome formulations for cosmetic application. Int J Pharm 296:44–54CrossRefPubMedGoogle Scholar
  11. 11.
    Taylor TM, Davidson PM, Bruce BD, Weiss J (2005) Liposomal nanocapsules in food science and agriculture. Crit Rev Food Sci Nutr 45:587–605CrossRefPubMedGoogle Scholar
  12. 12.
    Mozafari MR, Johnson C, Hatziantoniou S, Demetzos C (2008) Nanoliposomes and their applications in food nanotechnology. J Liposome Res 18:309–327CrossRefPubMedGoogle Scholar
  13. 13.
    Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder ML, Joung JK, Chen ZY, Liu DR (2015) Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat Biotechnol 33:73–80CrossRefPubMedGoogle Scholar
  14. 14.
    Lasic DD (1988) The mechanism of vesicle formation. Biochem J 256:1–11CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Lopez-Pinto JM, Gonzalez-Rodriguez ML, Rabasco AM (2005) Effect of cholesterol and ethanol on dermal delivery from DPPC liposomes. Int J Pharm 298:1–12CrossRefPubMedGoogle Scholar
  16. 16.
    Harries D, May S, Gelbart WM, Ben-Shaul A (1998) Structure, stability, and thermodynamics of lamellar DNA-lipid complexes. Biophys J 75:159–173CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Mislick KA, Baldeschwieler JD (1996) Evidence for the role of proteoglycans in cation-mediated gene transfer. Proc Natl Acad Sci U S A 93:12349–12354CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Lang JK, Vigo-Pelfrey C (1993) Quality control of liposomal lipids with special emphasis on peroxidation of phospholipids and cholesterol. Chem Phys Lipids 64:19–29CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.School of Pharmacy – BostonMCPHS UniversityBostonUSA

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