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Quantitative Efferocytosis Assays

  • Amanda L. Evans
  • Jack W. D. Blackburn
  • Charles Yin
  • Bryan Heit
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1519)

Abstract

Efferocytosis, the phagocytic removal of apoptotic cells, is a dynamic process requiring recruitment of numerous regulatory proteins to forming efferosomes in a tightly regulated manner. Herein we describe microscopy-based methods for the enumeration of efferocytic events and characterization of the spatiotemporal dynamics of signaling molecule recruitment to efferosomes, using genetically encoded probes and immunofluorescent labeling. While these methods are illustrated using macrophages, they are applicable to any efferocytic cell type.

Key words

Efferocytosis Phagocytosis Method Microscopy Immunostaining Macrophage 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Amanda L. Evans
    • 1
  • Jack W. D. Blackburn
    • 1
  • Charles Yin
    • 1
  • Bryan Heit
    • 1
  1. 1.Department of Microbiology and Immunology and the Centre for Human ImmunologyThe University of Western OntarioLondonCanada

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