Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages
Flow cytometry is a powerful analytical technique, which is increasingly being used to study the interaction between host cells and intracellular pathogens. Flow cytometry is capable of measuring a greater number of infected cells within a sample compared to alternative techniques such as fluorescence microscopy. This means that robust quantification of rare events during infection is possible. Our lab and others have developed flow cytometry methods to study interactions between host cells and intracellular pathogens, such as Cryptococcus neoformans, to quantify phagocytosis, intracellular replication, and non-lytic expulsion or “vomocytosis” from the phagosome. Herein we describe these methods and how they can be applied to the study of C. neoformans as well as other similar intracellular pathogens.
Key wordsCryptococcus neoformans Cryptococcus gattii Flow cytometry Macrophage Infection Mycology
- 3.Nicola AM, Robertson EJ, Albuquerque P, Derengowski Lda S, Casadevall A (2011) Nonlytic exocytosis of Cryptococcus neoformans from macrophages occurs in vivo and is influenced by phagosomal pH. MBio 2(4). doi:10.1128/mBio.00167,11. Print 2011
- 5.Alanio A, Desnos-Ollivier M, Dromer F (2011) Dynamics of Cryptococcus neoformans-macrophage interactions reveal that fungal background influences outcome during cryptococcal meningoencephalitis in humans. MBio 2(4). doi:10.1128/mBio.00158,11. Print 2011