Abstract
Generation of primary cell culture of hepatocytes by mouse liver perfusion (MLP) combines the advantages of in vivo and in vitro models. It provides hepatocytes that grow under physiological conditions in mice, with the genotype of the whole organism or a specific gene knockout. In contrast to immortalized cell cultures, primary murine hepatocytes (pmHep) are non-cancerous cells with a limited lifespan but still amenable to classical in vitro methods such as treatment with drugs, small molecule inhibitors, and agonistic/antagonistic antibodies of surface receptors as well as transfection. One technique, which has gained popularity recently, is the analysis of protein–protein interactions by the proximity ligation assay (PLA). Here, we describe a liver perfusion protocol and the detection of the histone deacetylase function of Sirtuin 6 (SIRT6) using PLA in pmHep.
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Acknowledgement
This work was supported by an Internal University Research Funding program of the Johannes Gutenberg-University Mainz, the Imaging Core Facility of the Research Center for Immunotherapy (FZI), and the International PhD Program of the Institute of Molecular Biology in Mainz.
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Wolf, K., Strand, S. (2017). Assessing the Histone Deacetylase Activity of SIRT6 in Primary Murine Hepatocytes via Proximity Ligation Assay. In: Krämer, O. (eds) HDAC/HAT Function Assessment and Inhibitor Development. Methods in Molecular Biology, vol 1510. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6527-4_11
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DOI: https://doi.org/10.1007/978-1-4939-6527-4_11
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